Monday, May 7, 2012
Well, we still don't have any results, nor have we begun the staining/freezing process because unlike last time, we need to grow a bunch more cells in order to have enough to freeze back. So we needed to grow them from the little twelve wells into the large flasks that we were using in the beginning. But we can't just transfer immediately into them, because there aren't enough to stimulate growth.We have to switch them from the small wells to a larger well to a small flask to the large flasks. The high infection cells are being switched to the large flasks tomorrow because they have a higher cell density the the low infection, only one of which has even been able to be transferred into the larger well. In conclusion, I'm going to continue going into TGen even though the scheduled internship is technically over so I can try to get some final results before the presentation is due. I also have been accepted as an Ivy Scholar which will give me a paid internship at TGen during the summer, so it's possible that I could finish the entire project and not just this portion. Maybe. I'll probably stop updating this though, because I have a bunch of other things I have to do and I'm pretty sure that internship is 40 hours a week.
Friday, May 4, 2012
Hey, so this week has been really slow as well. I've just been going in, switching the media for the infected cells and maintaining my HeLa cells, so I've only been going in for about an hour a day. Fortunately, the mock cells in the infected plate have all died out, which means that we're pretty much ready to freeze them back and stain them, which could happen as soon as this weekend. But I'm not sure, so this week might be a little short...
Subscribe to:
Posts (Atom)