On Thursday we concocted media (cell food) to put the cells in. Each line of cells required a different recipe of media, and the entire day was taken up by the project. Most of the media contained a base formula and a kind of serum either FBS (Fetal Bovine Serum) or Horse Serum. Combining the ingredients was a tedious process, and much ethanol was sprayed on gloves, packaged filters, and media containers in order to maintain sterility.
Friday was dedicated to thawing the cell lines that we needed and placing them in a preliminary media to be changed out the next day.
On Monday we checked on the cells to see if they were growing properly, and the PC-12 cells look like they're about ready to be split. Tomorrow will be short, but I will spend the majority of Wednesday and Thursday observing a western blot that we will eventually have to perform on our own cells.
Adios.
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