So, I am being tested on my ability to conduct procedures in a fume hood correctly, efficiently, and sterile-ly. To this end, I have been delegated a single flask of HELA cells to grow for a week in media with antibiotic in the media, then I have to grow them further without the antibiotic to see if I'm not messing up. Good news. I'm not messing up. I have been caring for these cells for quite a while now, and they are decidedly not dead. Just changed their media today, and they are showing a startling lack of contamination.
Also, we managed to actually seed two cell lines in a couple of 6-well plates like we were supposed to, and they should be ready to infect with virus tomorrow. After which I will tell how that went. Anyway, the only thing that really happened between what I told you about last time and finishing seeding the cells was we added more media so we had enough of a volume to out in each well, then we calculated how many mLs of the new solution would give us the proper number of cells per well, then we added them. And labelled them. The labeling was important.
We are going to kill all of the cells not infected by virus, too.
And some wells have more virus per cell, either .1 or .5 MOIs.
I think I already told you that.
But we can't just dump a whole bunch of ampicillin into the wells. That would kill the cells that even had the immunity from the virus. We have to acclimate the cells to the ampicillin's presence. Then, once the ampicillin has won the trust of the viral-cells, we will rampu up the dosage, killing all of the cells not infected.
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