So, I had to make more media today, since I have to keep growing cells because they suddenly decided that the middle of the flask was overrated. Also we have to thaw some more Neuro 2a and SY5Y cells because they basically did the same thing expect they decided the whole staying alive thing was just too much effort.

Yesterday we mummified PC12, HeLa, and H4 mock cells to measure PLAP levels against those that were infected. But we had to use the regular cells because all the mock cells that we had earlier in the infection process were exposed to the antibiotic. So they died. We also took the media from the infected PC12 cells for the RNA, DNA, and protein using the process that I now have the protocol for. This protocol comes with a kit with all the necessary parts.
So, according to the diagram in the protocol that won't paste into this thing, the cells get lysed in this tube, then put into a "Allprep column" and centrifuged so the DNA stays in the upper portion and the RNA and protein flows through. To purify the DNA, the top portion is washed and eluted (which means to remove an adsorbed substance by washing with a solvent). Then ethanol is added to the bottom part with the RNA and protein. Then it is placed in a "RNeasy column" and centrifuged to a point where the RNA remains in the top and the protein falls through. The part with the RNA is then washed and eluted and the bottom part with the precipitated protein is centrifuged and redissolved.