So yesterday and today I began the process of killing all of the cells that weren't infected with virus. It involved math and significant figures. I calculated the proper volume of antibiotic necessary for the low dose (on Saturday) and the high dose (today) when given the total volume of media I needed, the concentration of the antibiotic, and the needed dosage (in micrograms per milliliter). After the math, I actually had to make the antibiotic-media, which was pretty simple, I just put the proper amount of antibiotic into a 3.6 mL aliquot of media. Then I performed a basic media change on the cells, switching the normal media with the antibiotic one.

In other news I saw those stained slides of rat liver and heart and they were very pretty. I wish I had pictures, but I had left my image-taking-device upstairs. Also the liver had a lot more fibrosis than the heart. It was like a swirling vortex of red, purple, and blue whereas the heart was almost entirely red.

Tomorrow I'm going to switch the cells to the high dose and then keep them at that constant level until all of the cells in the wells with no infected cells die. I don't know how long it will take, probably a week, but then we can freeze some of them back and stain them and actually show that we infected everything properly and get a preliminary report on how much PLAP they are overproducing.