Sorry there has not been an update this week so far, it has been really slow/really busy. Slow in that the only thing I have been doing in the lab is switch the media in the infected cells to the high dosage of antibiotic and maintain it, busy in that I got a job at the theater and have not gotten home until late for the past few days (including today, actually).

The cells worried me for a bit because it did not look like any of them were dying. They had actually looked like they were growing too fast, but I continued the dosage as instructed and they pretty much all died within a 36 hour period. They now look normal with the mock cells at the lowest density, followed by the .1 MOI cells and then the 2.5 MOI cells have the most amount of cells still alive. I have to continue the high dose at a constant rate until all of the mock cells are dead to make sure that all of the cells that we stain are infected.

Just a quick recap of things present and soon to come: The media that the infected cells were in originally is what we are going to use to count the exosomes to determine the cell lines that produce the most and least exosomes, but we need to stain the cells themselves in order to show that we did the infection properly for both  antibiotic resistance and PLAP over-expression. Once we have confirmed the cells were properly infected (probably within the next week or so), we can count the exosomes in the media.

I wish I had something else to tell you, but there really hasn't been anything going on this week. I might be on for a paid summer internship continuing this project, but it has not been finalized yet.