So, yesterday we were able to infect the two cell lines from earlier in the week. It didn't take much, actually, we just emptied the wells of media, rinsed them, then put new media in which had a calculated amount of virus int them. We have to put the virus into the media before we put the media into the wells because it helps spread the virus more evenly over the well surface. If we put the media in first, the virus would be concentrated in what ever section we put it in. This way we maximize the number of cells infected with the virus to more closely approximate the number that we calculated.

Also, random note, everything that touches anything that might have had contact with the virus goes into bleach. Which we then let soak. Overnight. With the UV light on. It's not harmful, but we're not supposed to take chances. It might get into some other things that aren't supposed to have PLAP protein-making virus in them. 

Today I split my cells. They're still alive. But since today was a Saturday, no one else was in the lab. Sometimes people will come in to work on the weekends, but today there was absolutely no one. It was kind of awesome.