Update

Well, for the past week, we haven't been doing anything intensive. We have mostly just come in, checked the cells for growth then split them or changed the media if necessary. Today we put one of the cell lines into 6-well plates, and that was a different procedure because instead of measuring using volume, we had to get a specific number of cells into the well regardless of concentration. We had to use a cellometer to measure how many cells were in the solution, then we had to dilute it to the correct volume for the total volume that was going into the wells, then we actually put it in the wells.

I have to check on the cells everyday, and now we have to make special media to prevent the exosome count from going haywire because of the exosomes in the FBS that we have to put in the media. So we have to put the media in a special centrifuge that takes 16 hours to complete. So this part takes a lot of patience and timing.